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KMID : 0380020040190010038
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 1 p.38 ~ p.41
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Batch Production of Chiral Epichlorohydrin by Enantioselective Hydrolysis Reaction using Rhodosporidium toruloides
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Lee Eun-Yeol
Lee Jae-Hwa
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Abstract
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Enantioselective hydrolysis for the producing chiral epichlorohydrin from its racemic substrate was investigated using epoxide hydrolase activity of Rhodosporidium toruloides SJ-4. The effects of reaction parameters including pH, temperature, initial substrate concentration on initial hydrolysis rate and enantioselectivity were analyzed and optimized. The addition of detergent, Tween 20, enhanced the hydrolysis rate and enantioselectivity. Chiral (R)-epichlorohydrin with high optical purity (＞99% ee) and yield of 25% (theoretically 50% maximum yield) was obtained from its racemate of 20 mM.
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KEYWORD
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Enantioselective hydrolysis, epichlorohydrin, epoxide hydrolase, Rhodosporidium toruloides
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